| 质粒名称: | pGL4.0-TERT WT |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 84924 |
| 存储实验室: | Joseph Costello |
|
载体骨架基本信息 |
|
| 载体名称: | pGL4.0 |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TERT promoter |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | --- |
| -- | |
|
文献引用方法 |
|
| 材料和方法部分: | pGL4.0-TERT WT was a gift from Joseph Costello (Addgene plasmid # 84924 ; http://n2t.net/addgene:84924 ; RRID:Addgene_84924) |
| 参考文献部分: | Cancer. The transcription factor GABP selectively binds and activates the mutant TERT promoter in cancer. Bell RJ, Rube HT, Kreig A, Mancini A, Fouse SD, Nagarajan RP, Choi S, Hong C, He D, Pekmezci M, Wiencke JK, Wrensch MR, Chang SM, Walsh KM, Myong S, Song JS, Costello JF. Science. 2015 May 29;348(6238):1036-9. doi: 10.1126/science.aab0015. Epub 2015 May 14. 10.1126/science.aab0015 PubMed 25977370 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pGL4.0-TERT WT 4396 bp ds-DNA circular SYN 29-JUL-2019
DEFINITION luciferase reporter for TERT promoter.
ACCESSION .
VERSION .
KEYWORDS pGL4.0-TERT WT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4396)
AUTHORS Bell RJ, Rube HT, Kreig A, Mancini A, Fouse SD, Nagarajan RP, Choi
S, Hong C, He D, Pekmezci M, Wiencke JK, Wrensch MR, Chang SM, Walsh
KM, Myong S, Song JS, Costello JF
TITLE Cancer. The transcription factor GABP selectively binds and
activates the mutant TERT promoter in cancer.
JOURNAL Science. 2015 May 29;348(6238):1036-9. doi: 10.1126/science.aab0015.
Epub 2015 May 14.
PUBMED 25977370
REFERENCE 2 (bases 1 to 4396)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 29, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4396
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 33..81
/note="synthetic polyadenylation signal"
CDS 186..1046
/codon_start=1
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1246..1834
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1735..1754
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1988..2005
/label=L4440
/note="L4440 vector, forward primer"
polyA_signal 2253..2374
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2290..2309)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2344..2363
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
CDS complement(2418..4070)
/codon_start=1
/product="firefly luciferase"
/label=luciferase
/note="synthetic luc2 version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
regulatory 4067..4076
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 4324..19
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind complement(4356..4375)
/label=RVprimer3
/note="pGL3 vector, forward primer"
ORIGIN
1 ttgatggaga gcgtatgtta gtactatcga ttcacacaaa aaaccaacac acagatgtaa
61 tgaaaataaa gatattttat tgcggcctgt ccaatacctc ccgtacctta atattactta
121 cttatccttg agagacgtac tagtaaccct gataaatgct tcaataatat tgaaaaagga
181 cgagtatgag catccaacat tttcgtgtcg cactcattcc cttctttgcg gcattttgct
241 tgcctgtttt tgcacacccc gaaacgctgg tgaaagtaaa agatgctgaa gatcaactgg
301 gtgcaagagt gggctatatc gaactggatc tcaatagcgg caagatcctt gagtctttcc
361 gccccgaaga acgattcccg atgatgagca ctttcaaagt actgctatgt ggcgcggtgt
421 tgtcccgtat agacgccggg caagagcagc ttggtcgccg tatacactac tcacaaaacg
481 acttggttga gtactcgccg gtcacggaaa agcatcttac ggatggcatg acggtaagag
541 aattgtgtag tgctgccatt accatgagcg acaacaccgc ggccaactta cttctgacaa
601 cgatcggagg ccctaaggag ctgactgcat ttcttcataa tatgggtgat catgtgaccc
661 ggcttgaccg ctgggaacca gagttgaacg aagccatacc gaacgacgag cgtgatacca
721 cgatgccagt agcaatggcc acaactcttc ggaaactact cactggcgaa cttcttactc
781 tagcatcacg acagcagctc atagactgga tggaggcgga caaagtagca ggaccacttc
841 ttcgctcggc cctccctgct ggctggttca ttgctgacaa atcgggggcc ggtgaacgcg
901 gctctcgcgg catcattgct gcgctggggc ctgatggtaa gccctcacga atcgtagtga
961 tctacacgac ggggagtcag gccactatgg acgaacgaaa taggcagatc gctgagatcg
1021 gtgcctcact gatcaagcac tggtaaccac tgcagtggtt tagcatttgc ggccgctgtc
1081 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
1141 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
1201 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
1261 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
1321 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
1381 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
1441 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
1501 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
1561 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
1621 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
1681 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
1741 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
1801 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
1861 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
1921 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
1981 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc tgccggcacc tgtcctacga
2041 gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc
2101 ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacggatc cttatcgatt
2161 ttaccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
2221 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
2281 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
2341 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgctcgaag cggccggccg
2401 ccccgactct agaattatta cacggcgatc ttgccgccct tcttggcctt aatgagaatc
2461 tcgcggatct tgcgggcgtc caacttgccg gtcagtcctt taggcacctc gtccacgaac
2521 acaacaccac cgcgcagctt cttggcggtt gtaacctggc tggccacata gtccacgatc
2581 tccttctcgg tcatggtttt accgtgttcc agcacgacga ctgcggcggg cagctcgccg
2641 gcatcgtcgt cgggcaggcc ggcgaccccg gcgtcgaaga tgttggggtg ttgcagcagg
2701 atgctctcca gttcggctgg ggctacctgg tagcccttgt atttgatcag gctcttcagc
2761 cggtccacga tgaagaagtg ctcgtcctcg tcccagtagg cgatgtcgcc gctgtgcagc
2821 cagccgtcct tgtcgatgag agcgtttgta gcctcggggt tgttaacgta gccgctcatg
2881 atcatggggc cacggacgca cagctcgccg cgctggttca cacccagtgt cttaccggtg
2941 tccaagtcca ccaccttagc ctcgaagaag ggcaccacct tgcctactgc gccaggcttg
3001 tcgtcccctt cgggggtgat cagaatggcg ctggttgttt ctgtcaggcc gtagccctgg
3061 cggatgcctg gtaggtggaa gcgtttggcc acggcctcac ctacctcctt gctgagcggc
3121 gccccgccgc tggcgatctc gtgcaagttg cttaggtcgt acttgtcgat gagagtgctc
3181 ttagcgaaga agctaaatag tgtgggcacc agcagggcag attgaatctt atagtcttgc
3241 aagctgcgca agaatagctc ctcctcgaag cggtacatga gcacgacccg aaagccgcag
3301 atcaagtagc ccagcgtggt gaacatgccg aagccgtggt gaaatggcac cacgctgagg
3361 atagcggtgt cggggatgat ctggttgccg aagatggggt cgcgggcatg actgaatcgg
3421 acacaagcgg tgcggtgcgg tagggctacg cccttgggca atccggtact gccactactg
3481 ttcatgatca gggcgatggt tttgtcccgg tcgaagctct cgggcacgaa gtcgtactcg
3541 ttgaagccgg gtggcaaatg ggaagtcacg aaggtgtaca tgctttggaa gccctggtag
3601 tcggtcttgc tatccatgat gatgatcttt tgtatgatcg gtagcttctt ttgcacgttg
3661 aggatctttt gcagcccttt cttgctcacg aatacgacgg tgggctggct gatgcccatg
3721 ctgttcagca gctcgcgctc gttgtagatg tcgttagctg gggccacagc cacaccgatg
3781 aacagggcac ccaacacggg catgaagaac tgcaagctat tctcgctgca caccacgatc
3841 cgatggtttg tattcagccc atagcgcttc atagcttctg ccagccgaac gctcatctcg
3901 aagtactcgg cgtaggtaat gtccacctcg atatgtgcgt cggtaaaggc gatggtgccg
3961 ggcaccaggg cgtagcgctt catggctttg tgcagctgct cgccggcggt cccgtcttcg
4021 agtgggtaga atggcgctgg gcccttctta atgtttttgg catcttccat ggtggcttta
4081 ccaacagtac cggattgcca agcttggccg ccgaggccag atccagcgct gcctgaaact
4141 cgcgccgcga ggagagggcg gggccgcgga aaggaagggg aggggctggg agggcccgga
4201 gggggctggg ccggggaccc gggaggggtc gggacggggc ggggtccgcg cggaggaggc
4261 ggagctggaa ggtgaagggg caggactcga ggctagcgag ctcaggtacc ggccagttag
4321 gccagagaaa tgttctggca cctgcacttg cactggggac agcctatttt gctagtttgt
4381 tttgtttcgt tttgtt
//
